Biochemistry & Molecular Biology

By Dr. Sheetal Bhasin | Devi Ahilya Viswavidyalaya, Indore

Learners enrolled: 3601

Biochemistry is the branch of science that explores the chemical processes within and related to living organisms. Biochemistry focuses on processes happening at a molecular level inside our cells. It studies components like proteins, lipids and organelles. It also looks at how cells communicate with each other.

On the other hand, ‘Molecular Biology’ deals with a wide scope of issues related with molecular and cell biology, including structural and functional genomics, bioinformatics, molecular immunology, etc. ‘Genetic Engineering’ is a part of molecular biology. It is the direct manipulation of an organism’s genome, using biotechnology.

From this course learners will be able to understand about carbohydrates, lipids & vitamins. They will be able to discern the functioning of proteins nucleic acids & enzymes and comprehend about cell structure.

Learners will be able to understand about DNA & RNA and increase their comprehension about genetic coding. Learners will also be able to distinguish between DNA isolation in bacteria, plants and animals.

Summary

Course Status :	Completed
Course Type :	Elective
Language for course content :	English
Duration :	12 weeks
Category :	Biological Sciences & Bioengineering
Credit Points :	4
Level :	Undergraduate
Start Date :	08 Aug 2019
End Date :	31 Oct 2019
Enrollment Ends :	01 Sep 2019
Exam Date :	10 Nov 2019 IST

Note: This exam date is subject to change based on seat availability. You can check final exam date on your hall ticket.

Page Visits

Course layout

Weeks Weekly Lecture Topics

Week 1 Lecture 1 - STRUCTURE AND CLASSIFICATION OF CARBOHYDRATES

: Lecture 2 - STRUCTURE AND CLASSIFICATION OF LIPIDS

Lecture 3 - FAT SOLUBLE VITAMINS

Lecture 4 - WATER SOLUBLE VITAMINS

Week 2 : Lecture 5 - STRUCTURE OF PROTEINS

Lecture 6 - NUCLEIC ACID - DNA

Lecture 7 - RIBONUCLEIC ACID (R.N.A.)

Lecture 8 - TYPES OF IMMUNITY : INNATE IMMUNITY

Week 3 : Lecture 9 - TYPES OF IMMUNITY - ADAPTIVE IMMUNITY

Lecture 10 - PRIMARY AND SECONDARY IMMUNE RESPONSES

Lecture 11 - HUMORAL IMMUNITY

Lecture 12 - CELL MEDIATED IMMUNITY

Week 4 : Lecture 13 - ANTIGENS

Lecture 14 - ANTIBODIES

Lecture 15 - ANTIGEN ANTIBODY INTERACTION

Lecture 16 - IMMUNOTECHNIQUES

Week 5 : Lecture 17 - TUMOUR IMMUNOLOGY

Lecture 18 - ANIMAL CELL CULTURE

Lecture 19 - CELLS LINES AND GROWTH CURVE

Lecture 20 - TRANSFECTION OF CELL LINES

Week 6 : Lecture 21 - TRANSFECTION OF ANIMAL CELLS

Lecture 22 - ANTIBIOTIC RESISTANCE PART-I

Lecture 23 - ANTIBIOTIC RESISTANCE PART-II

Lecture 24 - STEM CELL CULTURE

Week 7 : Lecture 25 - APPLICATION OF STEM CELLS

Lecture 26 - STRUCTURE OF DNA

Lecture 27 - PROKARYOTIC DNA REPLICATION

Lecture 28 - EUKARYOTIC DNA REPLICATION

Week 8 : Lecture 29 - TRANSCRIPTION

Lecture 30 - RNA PROCESSING

Lecture 31 - TRANSPORTATION OF RNA OUT OF NUCLEUS

Lecture 32 - POLYADNYLATION AND SPLICING

Lecture 33 - TRANSPOSABLE ELEMENTS

Week 9 : Lecture 34 - GENETIC CODE AND WOBBLE HYPOTHESIS

Lecture 35 - PROKARYOTIC TRANSLATION

Lecture 36 - EUKARYOTIC TRANSLATION

Week 10 : Lecture 37 - PROKARYOTIC TRANSCRIPTION REGULATION

Lecture 38 - GENE REGULATION IN EUKARYOTES

Lecture 39 - ISOLATION OF DNA FROM BACTERIA

Lecture 40 - D.N.A. ISOLATION FROM PLANT & ANIMAL CELLS

Week 11 : Lecture 41 - CLONING VECTORS

Lecture 42 - ENZYMES USED IN RECOMBINANT DNA TECHNOLOGY

Lecture 43 - METHODS OF GENE TRANSFER

Lecture 44 - EXPRESSION AND DETECTION OF CLONES

Week 12 : Lecture 45 - BLOTTING TECHNIQUES

Lecture 46 - POLYMERASE CHAIN REACTION

Books and references

· B. D. Singh: “Biotechnology”, Kalyani Publishers.

· Brandon, C., and J. Tooze: “Introduction to Protein Structure”, Garland Publishing, New York/London, 1991.

· Brown T.A. (2006) : “ Gene Cloning and DNA Analysis: An Introduction”, 6th Edition, Wiley-Blackwell Scientific, John Wiley & Sons Ltd, The Atrium, Southern Gate, Chichster, West Sussex, UK.

· Brown T.A. (2006): “Genomes 3”, 3rd Edition, Garland Science Publishing, Taylor and Francis, New York and London.

· Burton E. Tropp: ‟ Molecular Biology Genes to Proteins”, 3rd Edition, Jones and Bartlett Publishers.

· Cooper, G.M. and Hausman, R.E. (2011):“The Cell: A Molecular Approach”, 5th edition, ASM Press & Sunderland, Washington, D.C. Sinauer Associates, MA.

· D. P. De Robertis (1965): ‟Cell Biology”, 4th edition, Saunders.

· David L. Nelson, Michael M. Cox: “Lehninger Principles of Biochemistry”, 4th edition, W. H. Freeman and Company, New York.

· De Robertis & De Robertis, Jr.: ‟Cell and Molecular Biology”, 8th Edition, Lippincott Williams & Wilkins, A Wolters Kluwer Company.

· De Robertis& De Robertis, Jr.: “Cell and Molecular Biology” 8th Edition, Lippincott Williams & Wilkins, A Wolters Kluwer Company.

· Gray, C. J.; Weissenborn, M. J.; Eyers, C. E.; Flitsch, S. L. (2013): "Enzymatic reactions on immobilised substrates", Chemical Society Reviews, 42 (15): 6378.

· Karp, G. (2010): “Cell and Molecular Biology: Concepts and Experiments”, 6th edition, John Wiley & Sons. Inc.

· L. Stryer: “Biochemistry”, W. H. Freeman and Company, San Francisco, 1975, Chapters 11-13.

· Michael M. Cox and David L. Nelson: “Lehninger’sPrinciples of Biochemistry”, 5th edn, W.H. Freeman and Company, 2010.

· P.K. Gupta: “Biotechnology”, Rastogi Publications.

· P.S. Verma ,V. K. Agrawal: ‟Cell Biology – Cytology, Biomolecules and Molecular Biology”, S. Chand And Company Pvt. Ltd.

· Robert K. Murray, Daryl K. Granner, Peter A. Mayes and Victor W. Rodwell: “Harper’s Illustrated Biochemistry”,26th edition, McGraw Hill Publications, 2003.

· S.C. Rastogi: “Cell and Molecular Biology”,2ndedition,New Age International.

· Thomas M. Devlin: “Textbook of Biochemistry with Clinical Correlations”, Seventh Edition, John Wiley and Sons Inc.

· Trevor Palmer and Philip Bonner: “Enzymes: Biochemistry, Biotechnology, Clinical Chemistry”, Second Edition, East West Press Private Limited, New Delhi.

· U. Satyanarayana, U. Chakrapani: “Biochemistry”, 3rd edition, Books and Allied (P) Ltd., Kolkata.

Instructor bio

Dr. Sheetal Bhasin is working as Professor at Department of Bio-sciences, Maharaja Ranjit Singh College of Professional Sciences, Indore. She holds a teaching experience of more than 20 years and research experience of more than 10 years. She is actively engaged in research on characterization, biodiversity, production of enzymes, primary and secondary metabolites from Actinomycetes. She has varied research publications and 47 conference presentations on production of bioactive compounds and enzymes by Actinomycetes at national and international level.