Plant Biochemistry and Plant Biotechnology

By Dr. K. Sanatombi   |   Assistant Professor, Department of Biotechnology, Manipur University
Learners enrolled: 1018

Plant biochemistry and biotechnology are two important branches of modern plant science. The course focuses on the biochemistry and biotechnology of plants.  The course is designed to give a brief understanding of the fundamentals of plant biochemistry with a view to provide the learners with an understanding of plant growth and requirements of plants.  Studying of biochemical processes including photosynthesis, respiration, nitrogen cycle, growth hormones and secondary metabolism will provide a unique insight into how plant cells can be used and manipulated in in vitro cultures. 

Plant tissue culture and genetic engineering are the two main techniques used in plant biotechnology. The course introduces the various aspects and application of plant tissue culture with a view to provide the fundamental knowledge of plant tissue culture to the learners. An attempt is also made to introduce gene transfer methods for plant transformation and also the applications of transgenic plants. The course winds up with a brief introduction of molecular markers and their application in crop improvement. 

Course Status : Completed
Course Type : Core
Duration : 12 weeks
Category :
Credit Points : 4
Level : Undergraduate
Start Date : 18 Jan 2021
End Date : 11 Apr 2021
Enrollment Ends : 28 Feb 2021
Exam Date :

Page Visits

Course layout

Week 1
: 1 Plant and animal cell
: 2 Photosynthesis: Significance of Photosynthesis
: 3 Ultrastructure of Chloroplast and photosynthetic pigments
: 4 Photosynthesis in C3 and C4 plants

Week 2
: 1 Photosynthetic light and dark reactions
: 2 Photophosphorylation and Photorespiration
: 3 Plant genome organization
: 4 Molecular biology of biotic and abiotic stresses

Week 3
: 1 Plant growth and development
: 2 Biological nitrogen fixation
: 3 Nitrate and sulphate reduction and their incorporation into amino acids
: 4 Secondary metabolites

Week 4
: 1 Secondary metabolites II
: 2 Plant hormones I: Introduction, biochemistry and mode of action of auxins
: 3 Plant hormones II: Gibberellins and Cytokinins
: 4 Plant hormones III: Ethylene and Abscisic Acid

Week 5
: 1 Concept of Plant Biotechnology
: 2 Totipotency and morphogenesis
: 3 Growth media, environmental factors and explants source
: 4 Micropropagation

Week 6
: 1 Embryo culture and plant organ culture
: 2 Callus culture and somatic embryogenesis
: 3 Single cell and cell suspension culture
: 4 Protoplast isolation, purification, culture and fusion

Week 7
: 1 Factors Affecting in vitro Secondary Metabolite Production 
: 2 Hairy Root Culture
: 3 Somaclonal Variation
: 4 Synthetic seed technology

Week 8
: 1 Germplasm Conservation
: 2 Direct and indirect methods of gene transfer
: 3 Transgenic plants and their applications
: 4 Morphological, Biochemical and DNA – based markers

Week 9
: 1 Marker assisted selection (MAS) and crop improvement
: 2 Requirements for plant tissue culture laboratory
: 3 Micropropagation
: 4 To Study about Callus Induction and Plant Regeneration

Week 10
: 1 Spectral analysis of DNA
: 2 Cell suspension culture
: 3 Determination of molecular size of DNA sample by agarose gel electrophoresis
: 4 DNA Profiling of Plants

Books and references

1. Taiz, L. and Zeiger, E. (2010) Plant Physiology. 5th Edition, Sinauer Associates, Inc., Sunderland.
2. Razdan, M. K. (2003). Introduction to plant tissue culture (2. ed. ed.). Enfield, NH [u.a.]: oxford
    Publishers. ISBN 1-57808-237-4.
3. Chawla, H.S. (2002). Introduction to plant biotechnology (2nd ed. ed.). Enfield, N.H.: Science
    Publishers. ISBN 1-57808-228-5.
4. Bhojwani, S.S. and Rajdan, Plant Tissue Culture: Theory and Practice, Amsterdam, Elsevier.
5. Roberta Smith, (2012) Plant Tissue Culture: Techniques and Experiments, Academic Press
6. Agriculture: Role of Genetic Engineering. Kluwer Academic Publishers, The Netherlands.
7. Basra, A.S. (1994). Srtess-induced Gene Expression in Plants. Taylor and Francis, London.
8. Basra, A.S. and Basra, R.K. (eds) (1997). Mechanism of Environmental Stress Resistance in Plants.
    Harwood Academic Publishers. The Netherlands.

Instructor bio

Dr. K. Sanatombi

Assistant Professor, Department of Biotechnology, Manipur University

Dr. K. Sanatombi is presently working as Assistant Professor in the Department of Biotechnology, Manipur University, since 2009. She holds more than nine years of post-graduate teaching experience in Biotechnology. Her main research interests are in the areas of Plant biotechnology, including in vitro secondary metabolite production and DNA profiling of medicinal and aromatic plants. She has published many research papers in international journals and contributed chapters in books published by international publishers.

Course certificate

30% for in course Assessment & 70% of end-term Proctored Exam

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