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Microbial Physiology and metabolism

By Dr. K. R. MARUTHI,   |   SDM College (Autonomous), Ujire, 574 240
Learners enrolled: 660
Microbes are ubiquitous and omnipresent. Understanding Microbes Physiology &their Metabolism provide insight knowledge on their sources of energy &its utilization as they are tiny factories for the production of high-value low-volume products to low-value high-volume products which are its primary & secondary metabolites. Primary metabolites are typically formed during the growth phase as a result of energy metabolism and are deemed essential for proper growth. And, many of the identified microbes’ secondary metabolites have a role in ecological function. Microbes have a diverse metabolic activity which not observed in any other group of organisms. Thus, it is critical understand Microbe’s physiology and metabolism, to manipulated them, in order to enhance their growth or to product desired products of commercial value, 

The syllabus is designed keeping in mind recent development in Microbial Physiology & Metabolism and will be useful to all those who want to learn and update their knowledge on this field.


Summary
Course Status : Ongoing
Course Type : Elective
Duration : 12 weeks
Start Date : 05 Jul 2021
End Date : 30 Oct 2021
Exam Date :
Enrollment Ends : 15 Sep 2021
Category :
  • Biological Sciences & Bioengineering
Credit Points : 4
Level : Undergraduate



Course layout


Weeks

Day

Weekly Lecture Topics

First Week

Day 1

Microbial enzymes: Structure and Classification

Day 2

Mechanism of Enzyme actions: Lock and key model, induced fit theory

Day 3

Factors affecting rates of enzyme mediated reactions

Day 4

The role of ATP in metabolism

Day 5

●       MCQ

●       Assignments

 

Second Week

Day 1

Definitions of growth and generation time, measurement of microbial growth and specific growth rate

Day 2

Batch and Continuous culture

Day 3

Phases and types of growth curve and its industrial application

Day 4

Microbial growth in response to temperature,  pH, solute and water activity, oxygen, pressure and radiation

Day 5

●       MCQ

●       Assignments

 

Third Week

Day 1

Classification of bacteria based on nutrients

Day 2

Membranes of microorganisms, Ion channels

Day 3

Passive and facilitated diffusion, Primary and secondary active transport, concept of uniport, symport and antiport

Day 4

Group translocation and Iron uptake

Day 5

●       MCQ

●       Assignments

 

Fourth Week

Day 1

Photosynthetic pigments and apparatus in bacteria

Day 2

Photophosphorylation

Day 3

C3 and C4 pathways

Day 4

Difference between oxygenic and anoxygenic photosynthesis

Day 5

●       MCQ

●       Assignments

Fifth Week

Day 1

Mode of nutrition in Hydrogen and Nitrifying bacteria

Day 2

Mode of nutrition in Purple sulfur bacteria, Non-sulfur bacteria and Green sulfur bacteria

Day 3

Mode of nutrition in methylotrophs and methanogens

Day 4

Utilization of light energy by halobacteria

Day 5

●       MCQ

●       Assignments

Sixth  Week

Day 1

Concept of aerobic respiration, anaerobic respiration and fermentation

Day 2

Sugar degradation pathways i.e., EMP, ED and Pentose phosphate pathway

Day 3

TCA cycle and Electron transport chain

Day 4

Comparison of mitochondrial and bacterial ETC, electron transport phosphorylation, uncouplers and inhibitors

Day 5

●       MCQ

●       Assignments

 

Seventh  Week

Day 1

Fates of pyruvate, Pasteur effect and industrial importance of fermentation

Day 2

Alcohol fermentation

Day 3

Lactate fermentation (homo fermentative and hetero fermentative pathways)

Day 4

Concept of linear and branched fermentation pathways

Day 5

●       Quizzes

●       Assignments

●       Paragraph and essay questions

 

Eighth Week

Day 1

Utilization of Lactose and Galactose

Day 2

Utilization of Maltose and Mannitol

Day 3

Degradation of cellulose, starch and glycogen

Day 4

Conversion of biomass to energy using microorganisms

Day 5

●       MCQ

●       Assignments

 

Ninth  Week

Day 1

Mechanism of nitrogen fixation

Day 2

Symbiotic and non symbiotic nitrogen fixation

Day 3

Biosynthesis of amino acids

Day 4

Degradation amino acids

Day 5

●       MCQ

●       Assignments

Tenth Week

Day 1

Oxidative stress

Day 2

Thermal stress

Day 3

Starvation stress and stringent response

Day 4

Aerobic to anaerobic transitions

Day 5

●       MCQ

●       Assignments

 

Eleventh   Week

Day 1

Exam preparation and assessment of assignments

Day 2

Day 3

Day 4

Day 5

Twelfth   Week

Day 1

Assessment at the end of the course, which comprises of 40% of Online or In-term assessment and 60% Proctored end term exam.

Day 2

Day 3

Day 4

Day 5

 

Books and references

1.   Moat A.G., Foster J.W. and Spector M.P. 2002. Microbial Physiology, 4th edition. A Johan Wiley and sons inc., publication.


2.  Kim B.H. and Gadd G.M. 2008. Bacterial physiology and metabolism. Cambridge University Press, Cambridge.


3.  Gilbert H.F. 2000. Basic concepts in biochemistry: A student’s survival guide. Second Edition. Mc-Graw-Hill Companies, health professions Division, New York.


4.  Madigan M.T., Martinko J.M., Stahl D.A. and Calrk D.P. 2012. Brock Biology of Microorganisms. 13th ed. Pearson Education Inc.


Instructor bio

Dr. K. R. MARUTHI,

SDM College (Autonomous), Ujire, 574 240
Dr. Katenahalli Rudrappa Maruthi is an academician who graduated from Kuvempu University with a Doctorate in Biotechnology 2002. He works as an Assistant Professor at Sri Dharmasthala Maanjunatheshwar College's Department of Biotechnology, with a total of 20 years of intensive teaching, research, and administrative experience. He is also the institution's Nodal Officer for NIRF ranking and coordinator for the NTC of NTA.

Dr. Maruthi served in the DRDO's (Defence Research and Development Organization) Field Research Laboratory in Leh, Ladakh, as a Research Associate. He was a part of two Biotechnology projects that focused on the indigenous flora of Ladakh. He spent seven years as a Principal in both Bangalore and Tirupati. He had organized two UGC sponsored and One Management sponsored National Conference. Published 25 peer-reviewed research articles in national and international publications. He had given the keynote address at several national and international conferences. He had led one Ph.D. and ten M.Phil. students to success. He is a member of three professional societies as a Life Member.

Course certificate

Assessment/Assignment marks will be considered for Internal Marks and will carry 30 percent for overall Result.

End Term Exam- will have 100 questions and will carry 70 percent of  overall Result.

*All students, who obtain 40% marks in in-course assessment and 40% marks in end-term proctored exam separately, will be eligible for certificate and credit transfer.


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