Microbial Physiology and metabolism

By Dr. K. R. MARUTHI, | SDM College (Autonomous), Ujire, 574 240

Learners enrolled: 660

Microbes are ubiquitous and omnipresent. Understanding Microbes Physiology &their Metabolism provide insight knowledge on their sources of energy &its utilization as they are tiny factories for the production of high-value low-volume products to low-value high-volume products which are its primary & secondary metabolites. Primary metabolites are typically formed during the growth phase as a result of energy metabolism and are deemed essential for proper growth. And, many of the identified microbes’ secondary metabolites have a role in ecological function. Microbes have a diverse metabolic activity which not observed in any other group of organisms. Thus, it is critical understand Microbe’s physiology and metabolism, to manipulated them, in order to enhance their growth or to product desired products of commercial value,

The syllabus is designed keeping in mind recent development in Microbial Physiology & Metabolism and will be useful to all those who want to learn and update their knowledge on this field.

Summary

Course Status :	Completed
Course Type :	Elective
Duration :	12 weeks
Category :	Biological Sciences & Bioengineering
Credit Points :	4
Level :	Undergraduate
Start Date :	05 Jul 2021
End Date :	30 Oct 2021
Enrollment Ends :	15 Sep 2021
Exam Date :

Page Visits

Course layout

Weeks	Day	Weekly Lecture Topics
First Week	Day 1	Microbial enzymes: Structure and Classification
	Day 2	Mechanism of Enzyme actions: Lock and key model, induced fit theory
	Day 3	Factors affecting rates of enzyme mediated reactions
	Day 4	The role of ATP in metabolism
	Day 5	● MCQ ● Assignments
Second Week	Day 1	Definitions of growth and generation time, measurement of microbial growth and specific growth rate
	Day 2	Batch and Continuous culture
	Day 3	Phases and types of growth curve and its industrial application
	Day 4	Microbial growth in response to temperature, pH, solute and water activity, oxygen, pressure and radiation
	Day 5	● MCQ ● Assignments
Third Week	Day 1	Classification of bacteria based on nutrients
	Day 2	Membranes of microorganisms, Ion channels
	Day 3	Passive and facilitated diffusion, Primary and secondary active transport, concept of uniport, symport and antiport
	Day 4	Group translocation and Iron uptake
	Day 5	● MCQ ● Assignments
Fourth Week	Day 1	Photosynthetic pigments and apparatus in bacteria
	Day 2	Photophosphorylation
	Day 3	C3 and C4 pathways
	Day 4	Difference between oxygenic and anoxygenic photosynthesis
	Day 5	● MCQ ● Assignments
Fifth Week	Day 1	Mode of nutrition in Hydrogen and Nitrifying bacteria
	Day 2	Mode of nutrition in Purple sulfur bacteria, Non-sulfur bacteria and Green sulfur bacteria
	Day 3	Mode of nutrition in methylotrophs and methanogens
	Day 4	Utilization of light energy by halobacteria
	Day 5	● MCQ ● Assignments
Sixth Week	Day 1	Concept of aerobic respiration, anaerobic respiration and fermentation
	Day 2	Sugar degradation pathways i.e., EMP, ED and Pentose phosphate pathway
	Day 3	TCA cycle and Electron transport chain
	Day 4	Comparison of mitochondrial and bacterial ETC, electron transport phosphorylation, uncouplers and inhibitors
	Day 5	● MCQ ● Assignments
Seventh Week	Day 1	Fates of pyruvate, Pasteur effect and industrial importance of fermentation
	Day 2	Alcohol fermentation
	Day 3	Lactate fermentation (homo fermentative and hetero fermentative pathways)
	Day 4	Concept of linear and branched fermentation pathways
	Day 5	● Quizzes ● Assignments ● Paragraph and essay questions
Eighth Week	Day 1	Utilization of Lactose and Galactose
	Day 2	Utilization of Maltose and Mannitol
	Day 3	Degradation of cellulose, starch and glycogen
	Day 4	Conversion of biomass to energy using microorganisms
	Day 5	● MCQ ● Assignments
Ninth Week	Day 1	Mechanism of nitrogen fixation
	Day 2	Symbiotic and non symbiotic nitrogen fixation
	Day 3	Biosynthesis of amino acids
	Day 4	Degradation amino acids
	Day 5	● MCQ ● Assignments
Tenth Week	Day 1	Oxidative stress
	Day 2	Thermal stress
	Day 3	Starvation stress and stringent response
	Day 4	Aerobic to anaerobic transitions
	Day 5	● MCQ ● Assignments
Eleventh Week	Day 1	Exam preparation and assessment of assignments
	Day 2
	Day 3
	Day 4
	Day 5
Twelfth Week	Day 1	Assessment at the end of the course, which comprises of 40% of Online or In-term assessment and 60% Proctored end term exam.
	Day 2
	Day 3
	Day 4
	Day 5

Books and references

1. Moat A.G., Foster J.W. and Spector M.P. 2002. Microbial Physiology, 4th edition. A Johan Wiley and sons inc., publication.

2. Kim B.H. and Gadd G.M. 2008. Bacterial physiology and metabolism. Cambridge University Press, Cambridge.

3. Gilbert H.F. 2000. Basic concepts in biochemistry: A student’s survival guide. Second Edition. Mc-Graw-Hill Companies, health professions Division, New York.

4. Madigan M.T., Martinko J.M., Stahl D.A. and Calrk D.P. 2012. Brock Biology of Microorganisms. 13th ed. Pearson Education Inc.

Instructor bio

Dr. K. R. MARUTHI,

SDM College (Autonomous), Ujire, 574 240

Dr. Katenahalli Rudrappa Maruthi is an academician who graduated from Kuvempu University with a Doctorate in Biotechnology 2002. He works as an Assistant Professor at Sri Dharmasthala Maanjunatheshwar College's Department of Biotechnology, with a total of 20 years of intensive teaching, research, and administrative experience. He is also the institution's Nodal Officer for NIRF ranking and coordinator for the NTC of NTA.

Dr. Maruthi served in the DRDO's (Defence Research and Development Organization) Field Research Laboratory in Leh, Ladakh, as a Research Associate. He was a part of two Biotechnology projects that focused on the indigenous flora of Ladakh. He spent seven years as a Principal in both Bangalore and Tirupati. He had organized two UGC sponsored and One Management sponsored National Conference. Published 25 peer-reviewed research articles in national and international publications. He had given the keynote address at several national and international conferences. He had led one Ph.D. and ten M.Phil. students to success. He is a member of three professional societies as a Life Member.

Course certificate

Assessment/Assignment marks will be considered for Internal Marks and will carry 30 percent for overall Result.

End Term Exam- will have 100 questions and will carry 70 percent of overall Result.

*All students, who obtain 40% marks in in-course assessment and 40% marks in end-term proctored exam separately, will be eligible for certificate and credit transfer.

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