Microbial Genetics

By Dr. Sudeshna Shyam Choudhury | St. Xavier's College (Autonomous), Kolkata

Learners enrolled: 698

This course on Microbial genetics will have discussions on the different basic aspects of genetics spread over 34 modules. The course content will include genome organization and different types, advantages, applications of mutation and mutagens. It will cover different types, host range, curing, replication, purification, incompatibility of Plasmids and the mechanism of genetic exchange among microbes by recombination and the process of conjugation, transformation and transduction. The genetics of T4 bacteriophage and that of Transposable elements in both prokaryotic and eukaryotic systems would also be discussed.

Summary

Course Status :	Completed
Course Type :	Core
Duration :	8 weeks
Category :	Biological Sciences & Bioengineering
Credit Points :	4
Level :	Undergraduate
Start Date :	10 Jan 2022
End Date :	31 Mar 2022
Enrollment Ends :	28 Feb 2022
Exam Date :

Page Visits

Course layout

Week 1

Module 01: Plasmid 1

Module 02: Plasmid 2

Module 03: Plasmid 3

Module 04: T4 bacteriophage

Week 2

Module 05: Transposons and transposition in prokaryotes

Module 06: Transposons and transpositions in eukaryotes

Module 07: Application of transposons in modern biology

Module 08: Model of recombination

Week 3

Module 9: Genetic analysis of recombination in bacteria

Module 10: Gene transfer in bacteria-transduction Part I

Module 11: Types of transduction: Generalized transduction

Modules 12: Specialized transduction

Week 4

Module 13: Bacterial transformation I

Module 14: Bacterial Transformation II

Module 15: Bacterial Conjugation I

Module 16: Bacterial conjugation II

Week 5

Module 17: Mutation: General introduction

Module 18: Different types of mutation

Module 19: Advantages and applications of mutations

Module 20: Mutagen I:Introduction to mutagen- General aspect

Week 6

Module 21: Mutagen II: Physical mutagens

Module 22: Mutagen III: Chemical mutagens

Module 23: Mutagen IV: Biological mutagen

Module 24: Mutagenesis

Week 7

Module 25: Level/Effect of mutation: at DNA sequence and gene level

Module 26: Biochemical level of mutations

Module 27: Effects of mutations

Module 28: Level of mutation: at chromosome level

Module 29: Effect of mutation at molecular implication level causing disorders

Week 8

Module 30:Reverse mutation—Back mutation and suppressor mutation

Module 31:Experiments related to mutation and reversion and mutation repair

Module 32:Transformation of E.coli (xl1-blue) cells by the plasmid DNA (puc-19)

Module 33:Induction of Enzyme Activity in Bacteria: The Lac Operon

Module 34: To learn the process of bacterial conjugation through the transfer of genes coding for antibiotic resistance

Books and references

1. Jones, S. 1993. The Language of the Genes. Harper Collins ISBN 0006552439.

2. Muller, H. J. (1927). "Artificial Transmutation of the Gene". Science 66 (1699)

3. Miller. J. (1992); A laboratory manual and handbook for Escherichia coli and related bacteria. CSHL Press, NY.

4. Genetic Engineering : First Edition : Oxford University Press: Smita Rastogi, Neelam Pathak

5. Principles of Gene Manipulation and Genomics: seventh edition: S.B. Primrose and R.M. Twyman.

6. Introduction to genetic analysis by Anthony J.F. Griffiths, Susan R. Wessler, Richard C. Lewontin, Sean B. Carroll. W.H. Freeman, 2008.

7. Modern Genetic Analysis.Griffiths AJF, Gelbart WM, Miller JH, et al.New York: W. H. Freeman; 1999.

8. Molecular genetics of bacteria by Larry Synder and Wendy Champness; 3rd edition, 2007.

9. Introduction to modern virology, 5th Edition by Dimmock NJ, Easton AJ, Leppard Keith; Blackwell Publishing (Indian reprint 2005).

10. Genetic Engineering : First Edition : Oxford University Press: Smita Rastogi, Neelam Pathak

Instructor bio

Dr. Sudeshna Shyam Choudhury

St. Xavier's College (Autonomous), Kolkata

Sudeshna Shayam Choudhury is the assistant professor in St.Xavier’s College, (Autonomous) Kolkata. She has done her Phd from Jadavpur University. The topic of her thesis was Cell Molecular Studies on Spontaneous Radiation namely, UV Stress Tolerance in Plants. She has done her post doctoral researches on several topics including, Plant Biochemistry and Biotechnology related to stress specially natural Ultraviolet stress on different altitude grown tea clones, Measurement of natural UV radiation in different altitudes, Effect of natural UV radiation in rice and tea, Genetic cataloguing of 2000 tea clones by DNA Fingerprinting method using RAPD, RFLP, ISSR, AFLP techniques, Microbial characterization by Biochemical, Morphological and Molecular Biological analysis, to name a few. Sudeshna has been the resource person for UGC sponsored lecture series (for 3 years) organized by EMMRC, Kolkata, and also for the in Skill Set Training in Science and technology, 2007, 2008, 2009, 2012—funded by D.B.T, Govt. Of India. She was also the resource person for Bose Institute workshop on hands-on-training regarding medicinal plants and DNA fingerprinting-funded by ICMR, for the years 2014, 2015. Sudeshna also has several publications in renowned journals to her credit. She has recently been awarded a project from National Tea Research Foundation, Tea Board Govt. Of India named “Studies on keeping quality of different types of tea (Black, Green, Oolong & White) and their biochemical aspects & antioxidant properties”.