Weeks 1
Day 1: Module 1- Introduction to Animal Biotechnology- Concept and Scope of Biotechnology
Day 2: Module 2 - Cloning vectors-I - Cosmids, Phagemids, Lambda Bacteriophage, M13, BAC, YAC, MAC
Day 3: Module 3 - Cloning vectors-II- Expression vectors
Day 4: Interaction\Discussion on Module 1, 2 & 3
Day 5: Assignments
Weeks 2
Day 1: Module 4- Restriction enzymes I
Day 2: Module 5 - Restriction enzymes types and detailed study of Type II
Day 3: Module 6 - Gene cloning
Day 4: Interaction\Discussion on Module 4, 5 & 6
Day 5: Assignments
Weeks 3
Day 1: Module 7- Gene transfer techniques
Day 2: Module 8 - Construction of genomic and cDNA libraries
Day 3: Module 9 - screening of DNA Libraries
Day 4: Interaction\Discussion on Module 7, 8 & 9
Day 5: Assignments
Weeks 4
Day 1: Module 10- Blotting techniques I (Southern and Northern)
Day 2: Module 11 - Blotting techniques II- Western blotting
Day 3: Module 12 - DNA sequencing
Day 4: Interaction\Discussion on Module 10, 11 & 12
Day 5: Assignments
Weeks 5
Day 1: Module 13- Polymerase Chain Reaction
Day 2: Module 14 - DNA Finger Printing
Day 3: Module 15 - DNA Micro array principle and technique.
Day 4: Interaction\Discussion on Module 13, 14 & 15
Day 5: Assignments
Weeks 6
Day 1: Module 16- Genetically modified organisms-I (Nuclear Transplantation)
Day 2: Module 17 - Genetically modified organisms-II (Retroviral Method)
Day 3: Module 18 - Genetically modified organisms-III (DNA microinjection)
Day 4: Interaction\Discussion on Module 16, 17 & 18
Day 5: Assignments
Weeks 7
Day 1: Module 19- Transgenic Animals
Day 2: Module 20 - Applications of transgenic animals I- Production of Pharmaceuticals
Day 3: Module 21- Applications of transgenic animals: production of donor organs
Day 4: Interaction\Discussion on Module 19, 20 & 21
Day 5: Assignments
Weeks 8
Day 1: Module 22- Applications of transgenic animals: knock out mice
Day 2: Module 23 - Production of transgenic plants- Agrobacterium mediated transformation
Day 3: Module 24 - Applications of transgenic plants: Insect and herbicide resistant plants
Day 4: Interaction\Discussion on Module 22, 23 & 24
Day 5: Assignments
Weeks 9
Day 1: Module 25- Animal cell culture
Day 2: Module 26 - Expressing cloned genes in mammalian cells
Day 3: Module 27 - Diagnosis of genetic diseases (Cystic fibrosis, Sickle cell anemia)
Day 4: Interaction\Discussion on Module 25, 26 & 27
Day 5: Assignments
Weeks 10
Day 1: Module 28- Recombinant DNA in medicine I- Recombinant insulin & Human growth hormone
Day 2: Module 29 – Gene Therapy
Day 3: Module 30 - Animal propagation-– Artificial insemination
Day 4: Interaction\Discussion on Module 28, 29 & 30
Day 5: Assignments
Weeks 11
Day 1: Module 31- Conservation Biology I- In vitro fertilization and embryo transfer in humans
Day 2: Module 32 - Conservation Biology II- In vitro fertilization and embryo transfer in farm animals
Day 3: Module 33 - Animal diseases need help of Biotechnology- Foot-and-mouth disease
Day 4: Interaction\Discussion on Module 31, 32 & 33
Day 5: Assignments
Weeks 12
Day 1: Module 34- Genomic DNA isolation from E. Coli-Practical
Day 2: Module 35 - Plasmid DNA isolation- Practical
Day 3: Module 36 - Restriction digestion- Practical
Day 4: Interaction\Discussion on Module 34, 35 & 36
Day5: Term end assessment
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