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ANIMAL BIOTECHNOLOGY

By Dr. JAYASREE. S   |   Mercy College, Palakkad
Learners enrolled: 4009
Animal biotechnology is a branch of biotechnology in which molecular biology techniques are used to genetically engineer animals in order to improve their suitability for agriculture, industrial and pharmaceutical applications. Advances in animal biotechnology have been facilitated by recent progress in sequencing animal genomes, gene expression and metabolic profiling of animal cells. Genome editing technologies (Zinc Finger Nucleases, TALENS, and CRISPR-Cas systems) have opened up new opportunities to easily create genetic variations in animals that can improve their health and well-being, agricultural production, and protection against diseases. The objective of this course is to familiarize the techniques involved animal biotechnology. The course aims to provide theory and practical sessions of biotechnology as part of Professionalization. The course can be well utilized for students, teachers or public those who have aspiration to learn modern science which keeps the world today. 
Summary
Course Status : Ongoing
Course Type : Elective
Duration : 12 weeks
Start Date : 17 Jan 2022
End Date : 18 Apr 2022
Exam Date :
Enrollment Ends : 28 Feb 2022
Category :
  • Biological Sciences & Bioengineering
Credit Points : 4
Level : Undergraduate

Page Visits



Course layout


Weeks 1
Day 1: Module 1- Introduction to Animal Biotechnology- Concept and Scope of Biotechnology
Day 2: Module 2 - Cloning vectors-I - Cosmids, Phagemids, Lambda   Bacteriophage, M13, BAC, YAC, MAC
Day 3: Module 3 - Cloning vectors-II- Expression vectors
Day 4: Interaction\Discussion on Module 1, 2 & 3
Day 5: Assignments

Weeks 2
Day 1: Module 4- Restriction enzymes I
Day 2: Module 5 - Restriction enzymes types and detailed study of Type II
Day 3: Module 6 - Gene cloning
Day 4: Interaction\Discussion on Module 4, 5 & 6
Day 5: Assignments

Weeks 3
Day 1: Module 7- Gene transfer techniques
Day 2: Module 8 - Construction of genomic and cDNA libraries
Day 3: Module 9 - screening of DNA Libraries
Day 4: Interaction\Discussion on Module 7, 8 & 9
Day 5: Assignments

Weeks 4
Day 1: Module 10- Blotting techniques I (Southern and Northern)
Day 2: Module 11 - Blotting techniques II- Western blotting
Day 3: Module 12 - DNA sequencing
Day 4: Interaction\Discussion on Module 10, 11 & 12
Day 5: Assignments

Weeks 5
Day 1: Module 13- Polymerase Chain Reaction
Day 2: Module 14 - DNA Finger Printing
Day 3: Module 15 - DNA Micro array principle and technique.
Day 4: Interaction\Discussion on Module 13, 14 & 15
Day 5: Assignments

Weeks 6
Day 1: Module 16- Genetically modified organisms-I (Nuclear Transplantation)
Day 2: Module 17 - Genetically modified organisms-II (Retroviral Method)
Day 3: Module 18 - Genetically modified organisms-III (DNA microinjection)
Day 4: Interaction\Discussion on Module 16, 17 & 18
Day 5: Assignments

Weeks 7
Day 1: Module 19- Transgenic Animals
Day 2: Module 20 - Applications of transgenic animals I- Production of Pharmaceuticals
Day 3: Module 21- Applications of transgenic animals: production of donor organs
Day 4: Interaction\Discussion on Module 19, 20 & 21
Day 5: Assignments

Weeks 8
Day 1: Module 22- Applications of transgenic animals: knock out mice
Day 2: Module 23 - Production of transgenic plants- Agrobacterium mediated transformation
Day 3: Module 24 - Applications of transgenic plants: Insect and herbicide resistant plants
Day 4: Interaction\Discussion on Module 22, 23 & 24
Day 5: Assignments

Weeks 9
Day 1: Module 25- Animal cell culture
Day 2: Module 26 - Expressing cloned genes in mammalian cells
Day 3: Module 27 - Diagnosis of genetic diseases (Cystic fibrosis, Sickle cell  anemia)
Day 4: Interaction\Discussion on Module 25, 26 & 27
Day 5: Assignments

Weeks 10
Day 1: Module 28- Recombinant DNA in medicine I- Recombinant insulin & Human growth hormone
Day 2: Module 29 – Gene Therapy
Day 3: Module 30 - Animal propagation-– Artificial insemination
Day 4: Interaction\Discussion on Module 28, 29 & 30
Day 5: Assignments

Weeks 11
Day 1: Module 31- Conservation Biology I- In vitro fertilization and embryo transfer in humans
Day 2: Module 32 - Conservation Biology II- In vitro fertilization and embryo transfer in farm animals
Day 3: Module 33 - Animal diseases need help of Biotechnology- Foot-and-mouth disease
Day 4: Interaction\Discussion on Module 31, 32 & 33
Day 5: Assignments

Weeks 12
Day 1: Module 34- Genomic DNA isolation from E. Coli-Practical
Day 2: Module 35 - Plasmid DNA isolation- Practical
Day 3: Module 36 - Restriction digestion- Practical
Day 4: Interaction\Discussion on Module 34, 35 & 36
Day5: Term end assessment

Books and references

1. Primrose S.B. and R.M. Twyman. Principles of Gene manipulation and Genomics. 7th Ed.2006 Blackwell Publishing 667p
2. Sandy B. Primrose, Richard Twyman, Bob Old. Principles of Gene Manipulation: An Introduction to Genetic Engineering. 6th Ed.2001 Wiley
        Blackwell Publishing 667p
3. T. A. Brown: Gene Cloning and DNA Analysis: An Introduction. 8th Ed.2020.Wiley-Blackwellpubling 395p.
4. Monika Jain. Recombinant DNA Techniques: A Textbook.2012. Alpha Science International Ltd; 1st edition,288pp.
5. James D Watson, Michael Gilman, Jan Witkowski and Mark Zoller. Recombinant DNA .2nded. Scientific American Books, New York. 1992.
        626pp.
6. Julia Lodge, Peter Lund, Steve Minchin. Gene Cloning
7. 1st Edition. Taylor & Francis 474pp.
8. Richard J. Reece. Analysis of Genes and Genomes, 2004. John Wiley & Sons Inc, 490pp.
9. M.M. Ranga Animal Biotechnology 3rd Ed.2007.  Agrobios India, 210pp.
10. A.K. Srivastava. Animal Biotechnology.2018. Oxford & IBH Publishing Co Pvt.Ltd,458pp.
11. B. Singh , S.K. Gautam .Textbook of Animal Biotechnology.2013.Oxford & IBH Publishing Co Pvt.Ltd,620pp.
12. John Masters. Animal Cell Culture:2000. A Practical Approach. OUP Oxford,334pp.
13. T. Satyanarayana, Bhavdish Narain Johri, Anil Prakash. 2012.Microorganisms in Sustainable Agriculture and Biotechnology.  Springer 
         publishing, 850 pp.
14. Jogdand, S.N. Gene Biotechnology,2019. Himalaya Publishing House.4thEd.447pp.
15. Joshi, P. Genetic Engineering and Its Applications. 2006.Agrobios India.328pp.

Instructor bio

Dr. JAYASREE. S

Mercy College, Palakkad
Dr.Jayasree.S is currently working as  Associate Professor in the  Department of Zoology, Mercy College, Palakkad, Kerala, India.  She has done Masters in Zoology, M.Phil in Zoology and Ph.D in Biotechnology from Department of Biotechnology, Cochin University of Science and Technology. She has 27 years of teaching and research experience in the areas of Biotechnology. She has been working as coordinator of Biotechnology and Bioinformatics courses at Mercy College Palakkad for more than 20 years. She is also serving as a resource person for introducing Biotechnology and Bioinformatics techniques through seminars and hands on training to the students and teachers. Her goal is to give best to the student community.

Course certificate

30% weightage internal assessment and 70% weightage main examination


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