Nutritional and Clinical Biochemistry

By Dr. Senjam Sunil Singh   |   Department of Biochemistry, Manipur University
Learners enrolled: 2010
After the completion of the course the students will have a better understanding on chemical properties of nutrients and other dietary constituents with scientific approach by describing their functions at cellular and molecular levels.
The course will also provide an in-depth knowledge about how a clinical investigation of laboratory tests in humans (such as body fluids) are used for diagnosis of diseases and its therapeutic purposes.


Course Status : Ongoing
Course Type : Core
Duration : 12 weeks
Start Date : 23 Jan 2023
End Date : 02 Apr 2023
Exam Date :
Enrollment Ends : 15 Mar 2023
Category :
  • Chemistry
Credit Points : 4
Level : Undergraduate

Page Visits

Course layout

  1. Introduction and definition of food and nutrition,
  2. Basic food groups: energy yielding, body building and protective foods,
  3. Physiology and nutrition of carbohydrates and fats,
  4. Physiology and nutrition of proteins and water.

  1. Fat soluble vitamins: Vitamin A, D, E, K,
  2. Water soluble vitamins: vitamin B complex and vitamin C,
  3. Minerals (Ca, Fe and iodine) and their biological functions,
  4. Composition of balanced diet and recommended dietary allowance (RDA).

  1. Basic concepts of energy expenditure, units of energy,
  2. The basal metabolic rate (BMR) and its determination,
  3. Specific dynamic action (SDA) of foods,
  4. Planning of dietary regimes for infants, pregnancy and old age,

  1. Nutrition and brain development,
  2. Mother’s milk versus formulated milk feeding,
  3. Food adulterations and precautions
  4. Basic concepts of Clinical Biochemistry,

  1. Types and uses of biochemical tests,
  2. Clinical Biochemistry laboratory
  3. Specimen collection, processing and transportation,
  4. Specimen collection, processing and transportation-II,

  1. Blood cell count & anemia,
  2. Hemophilia
  3. Diseases related to carbohydrate metabolism,
  4. Diabetes mellitus,

  1. Diseases related to amino acid and nucleic acid metabolism,
  2. Phenylketonuria, hypo- and hyperuricemia,
  3. Diseases related to lipid metabolism,
  4. Liver Function tests,

  1. Metabolism of bilirubin,
  2. Jaundice,
  3. The Kidneys
  4. Clinical enzymology,

  1. Isozymes and diagnostic tests,
  2. Enzyme patterns in acute pancreatitis, liver damages and myocardial infarction,
  3. Enzymes used in cancer therapy,
  4. Enzyme Regulation

WEEK 10:
  1. To determine the effect of substrate concentration and the effect of temperature on the enzyme (urease) activity 
  2. Separation of proteins by salt precipitation and ion exchange chromatography
  3. Enzyme Immobilisation of Urease from Cajanus cajan in Calcium Alginate
  4. Estimation of glucose, urea, creatinine and albumin in urine

Books and references

1. Tom Brody: Nutritional Biochemistry, Academic Press.

2. J. M. Deman: Principles of Food Chemistry, Aspen Publishers, Inc. 

3. Gerald F., Jr Combs: The Vitamins: Fundamental Aspects in Nutrition and Health, Academic Press 

4. Lester Packer (Editor), et al.: Antioxidant Food Supplement in Human Health, Academic Press 

5. Andreas M. Papas(Editor), James H. Quillen: Antioxidant Status, Diet, Nutrition, and Health, CRC Press 

6. Marc Hillbrand (Editor) and Reuben T. Spitz (Editor): Lipids, Health, and Behavior, American Psychological Association (APA) 

7. Thomas M. Devlin (Editor): Textbook of Clinical Correlations, John Wiley & Sons John Phd Baynes(Editor), Marek H. Dominiczak: Medical Biochemistry, Mosby-Year Book 

8. Dawn B. Marks, et al.: Basic Medical Biochemistry: A Clinical Approach, Lippincott, Williams & Wilkins 

9. Paul F. Torrence (Editor): Biomedical Chemistry: Applying Chemical Principles to the Understanding and Treatment of Disease, John Wiley & Sons 

10. Richard F. Luduena: Learning More Biochemistry: 100 New Case-Oriented Problems, Wiley-Liss 

11. George Kaldor: Clinical Enzymology 

12. Moselio Schaechter (Editor): Mechanisms of Microbial Disease, Lippincott, Williams & Wilkins 

13. Natalie Angier, et al.: Natural Obsessions: Striving to Unlock the Deepest Secrets of the Cancer Cell, Houghton Mifflin Co (Pap) 

14. Jay A. Levy: HIV and the Pathogenesis of AIDS, American Society for Microbiology 

15. E. Hooper and W. D. Hamilton: The River: A Journey to the Source of HIV and AIDS, Back Bay Books 

16. J. Cohen: Shots in the Dark: The Wayward Search for an AIDS Vaccine, W.W. Norton & Company 

17. John C. Reed (Editor): “Apoptosis” in Methods in Enzymology, Vol. 322, Academic Press 

18. William R. Clark: A Means to an End: The Biochemical basis of aging and Death, Oxford Univ Press 

19. G. C. Brown (Editor), et al.: Mitochondria and Cell Death, Princeton University Press

Instructor bio

Dr. Senjam Sunil Singh

Department of Biochemistry, Manipur University
Dr. Senjam Sunil Singh is an Associate Professor, Biochemistry, Manipur University, India. He received his PhD from the same University. After his nine years of teaching in the College, he joins the University. He got the DBT Oversea Associateship and under the same program he did his post-doctoral research training at one of the renowned research laboratory under Prof. Tzi Bun Ng, CUHK.  He is a regular contributor of research articles in his area and as reviewer for some peer research journals. At present he is working on protein biochemistry, especially purification and biochemical characterization of plant lectins. His area of research interest includes Lectin Biochemistry, Enzyme Biotechnology and Immunology.

Course certificate

30% for in-course Assessment & 70% for end-term Proctored Exam.

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