Basic Concepts in Enzymology

By Dr. Deepa G Muricken   |   St. Mary’s College Thrissur
Learners enrolled: 254
Enzymes are pivotal part of living system. They are biological catalysts that perform myriads of metabolic reactions that sustain life. Enzymes facilitate life process in all life forms ranging from prokaryotes to eukaryotes. Hence the study of enzymes is crucial in understanding biochemical basis of life.
This course on ‘Basic concepts in Enzymology’ is designed to introduce students to various theoretical and practical aspects of enzymology. This course will cover topics such as Enzyme classification and nomenclature, mechanism of enzyme action and the factors affecting their activities, enzyme kinetics, enzyme inhibition, regulation of enzyme activity, isolation and purification of enzymes and various industrial and clinical applications of enzymes. The course will consist of lectures, tutorials and interaction/discussion sections.   
This is an introductory course intended for undergraduate students in Biochemistry, Biotechnology, Microbiology and other life science courses including Botany, Zoology etc. Basic understanding about the biological catalysts can open new avenues for interdisciplinary research. Further, this course would serve as foundation for advanced Enzymology studies.

Course Status : Upcoming
Course Type : Core
Duration : 12 weeks
Category :
  • Biological Sciences & Bioengineering
Credit Points : 4
Level : Undergraduate
Start Date : 08 Jul 2024
End Date : 25 Sep 2024
Enrollment Ends : 31 Aug 2024
Exam Date : 15 Dec 2024 IST
Exam Shift :

Shift 2

Note: This exam date is subjected to change based on seat availability. You can check final exam date on your hall ticket.

Page Visits

Course layout

Weeks Weekly Lecture Topics
Week 1
1. Module 1 Introduction to enzymes
2. Module 2 Overview of enzyme function in a cell
3. Module 3 Structural organization of Enzymes

Week 2
1. Module 4 Nature of Enzymes I Catalytic power and specificity: Optical, geometrical, absolute, group, bond specificities
2. Module 5 Nature of Enzymes II- Coenzymes
3. Module 6 Nature of Enzymes III

Week 3
1. Module 7 Classification and Nomenclature of Enzymes I
2. Module 8 Classification of Enzymes II
3. Module 9 Classification of Enzyme III

Week 4
1. Module 10 Module 10 Module Units of Enzyme acivity
2. Module 11 Factors affecting Enzyme activity I
3. Module 12 Factors affecting Enzyme activity II

Week 5
1. Module 13 Kinetic analysis of Enzyme catalysed reactions I
2. Module 14 Kinetic analysis of Enzyme catalysed reactions II
3. Module 15 Enzyme Inhibition I

Week 6
1. Module 16 Enzyme inhibition II
2. Module 17 Irreversible inhibition
3. Module 18 Enzyme substrate interactions

Week 7
1. Module 19 Protease Family of Enzymes
2. Module 20 Mechanism of Enzyme catalysis I
3. Module 21 Mechanism of Enzyme catalysis II

Week 8
1. Module 22 Regulation of Enzymes: Allosteric Enzymes I
2. Module 23 Allosteric Enzymes II
3. Module 24 Zymogen activation of Enzymes

Week 9
1. Module 25 Covalent modification of Enzymes
2. Module 26 Isoenzymes
3. Module 27 Multienzyme complexes

Week 10
1. Module 28 Industrial application of Enzymes
2. Module 29 Clinical application of Enzymes
3. Module 30 Inborn errors due to Enzyme deficiency

Week 11
1. Module 31 Enzymology of biologically important amines
2. Module 32 Purification of Enzymes I
3. Module 33 Purification of Enzymes II

Week 12
1. Module 34 Purification of Enzymes III
2. Module 35 Recent advances in Enzymology
3. Module 36 Conclusion

Books and references

1. Biochemistry. 5th edition, Berg JM, Tymoczko JL, Stryer L. publisher: W H Freeman
2. Biochemistry, 2nd edition Reginald Garrett and Charles Grisham
3. Enzymes 2nd Edition Biochemistry, Biotechnology, Clinical Chemistry by T Palmer P L Bonner
4. Color Atlas of Biochemistry 2nd edition by Jan Koolman and Klaus-Heinrich Roehm
5. Cornish-Bowden, A., Fundamentals of Enzyme Kinetics (revised ed.), Portland Press (1995). [A lucid and detailed account of enzyme kinetics.]
6. Nelson, David L. (David Lee), 1942-. Lehninger Principles of Biochemistry. New York :W.H. Freeman, 2005.
7. Voet, D., Voet, J. G., & Pratt, C. W. (2008). Fundamentals of biochemistry: Life at the molecular level. Hoboken, NJ: Wiley.
8. Tymoczko, LubertStryer,and LubertStryer. Biochemistry. New York: W.H. Freeman, 2002. Print.

Instructor bio

Dr. Deepa G Muricken

St. Mary’s College Thrissur
Dr. Deepa G Muricken Asst. Professor in Biochemistry, St. Mary’s College Thrissur done her PhD at Protein Science and Technology, CFTRI Mysore. During PhD work she worked on engineering Enzyme Inhibitors and analyzing them. She has research experience at Indian Institute of Science Bangalore and worked as research scientist at Monsanto research Centre Bangalore. She moved to teaching field for the past 9 years and has several funded projects and publications.

Course certificate

Course Completion will carry 70% weightage of end term exam and 30% weightage of interal assessments. A minimum 40% in each is required to qualify for the Course Completion Certifcate.

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