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Advanced Microbiology

By Dr. Arup Kumar Mitra   |   St Xavier's College, Kolkata
Learners enrolled: 3793

The Advanced Microbiology is a 33 module course on advanced microbiology, which will equip the students with certain advanced aspects of basic microbiological techniques that include detailed classification of viruses, yeast and fungi. Production of antibiotics and their effect on the normal and pathogenic microflora of human body. Certain beneficial aspects of soil bacteria in increasing the soil fertility are also considered here. The most attractive feature of this module is that there are 15 modules of practical microbiology, which include preparation of culture media for different microbes, handling of different organisms, sterilization techniques, and isolation of microbes from nature, biomass measurement, and antibiotic sensitivity of microbes. Successful completion of this module will enable a student to perform practical hands on basic microbiological techniques that can be quite handy whenever he or she joins any research laboratory or even in industry.

Summary
Course Status : Ongoing
Course Type : Core
Language for course content : English
Duration : 12 weeks
Category :
  • Biological Sciences & Bioengineering
Credit Points : 4
Level : Undergraduate
Start Date : 15 Jul 2024
End Date : 31 Oct 2024
Enrollment Ends : 31 Aug 2024
Exam Date : 15 Dec 2024 IST
Exam Schedule :

Shift 1

Note: This exam date is subject to change based on seat availability. You can check final exam date on your hall ticket.


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Course layout

Week1:
Module 1: Morphology, Disease and Holmes Classification.
Module 2: Classification of viruses-I
Module 3: Classification of viruses II

Week 2:
Module 4: Classification of fungi: Classical Concept -I
Module 5: Classification of fungi: Classical Concept –II

Week 3:
Module 6: Modern Classification of fungi: Modern Concept - I
Module 7: Classification of fungi: Modern Concept – II
Module 8: Classification of fungi: Modern Concept – III

Week 4:
Module 9: Classification of yeast-I
Module 10: Classification of yeast-II

Week 5:
Module 11: Application of microbes
Module 12: Antibiotics: The secondary Metabolites
Module 13: Role of Staphylococcus in infection

Week 6:
Module 14: Nitrogen fixation & Role of Nif and Nod genes.
Module15: Microbiology & Human Health
Module 16: Bacterial Diseases

Week 7:
Module 17: The pathogenesis of Vibrio cholera
Module 18: Introduction to Salmonella
Module 19: Preparation of Culture Media: Liquid Medium

Week 8:
Module 20: Preparation of Culture Media: Solid Medium (Practical)
Module 21: Sterilization Techniques (Practical)
Module 22: Isolation of single colony on solid media (Practical)

Week 9:
Module 23: Enumeration of bacterial numbers by serial dilution & plating (Practical)
Module 24: Measurement of fungal bio-mass (Practical)
Module 25: Animal Handling (Practical)

Week 10:
Module 26: Staphylococcus aureus
Module 27: Isolation of protozoa from soil
Module 28: Antibiotic sensitivity assay: natural products

Week 11:
Module 29: Preparation of culture media for pathogenic bacteria – part 1
Module 30: Preparation of culture media for pathogenic bacteria – part 2

Week 12:
Module 31: Albert and Giemsa Staining
Module 32: Determination of Growth Phase of E. coli by Measurement of OD & cfu
Module 33: Preparation of Buffer Solution

Books and references

1. Michael T. Madigan John M. Martinko David A. Stahl David P. Clark, Brock; ‘Biology of Microoganisms’ (2009) Pearson.

2. S.J. Flint, L.W. Enquist ,R.M. Krug,V.R. Racaniello, A.M. Skalka; ‘Principles of Virology: Molecular Biology, Pathogenesis and Control’.

3. King, A.M.Q., Adams, M.J., Carstens, E.B. and Lefkowitz, E.J. San Diego (Ed.); ‘Virus taxonomy: classification and nomenclature of viruses: Ninth Report of the International Committee on Taxonomy of Viruses’; Elsevier, 2012.

4. Jeffrey c. Pommerville; ‘Alcamo’s Fundamentals of Microbiology’ (2007) Jones and Bartlett Publishers.

Instructor bio

Dr. Arup Kumar Mitra has a teaching experience of 24 years teaching Microbiology, Botany and Environmental Science. He is an associate Professor and founder Head of the P.G. Department of Microbiology, St. Xavier’s College, Kolkata. He is a Ph.D in Botany from Calcutta University. Recipient of National Merit Scholarship from Govt. of India. He has post -doctoral research experience from South Bank University, London. He is a Fellow of Indian Botanical Society and the International Society for Conservation of Nature and Natural Resources. He has more than 30 books in Biological Science, Environmental Science and Microbiology and has published more than 70 papers in referred National and International Journals. He has completed five research projects sponsored by UGC, DST. Five students are presently working under him for their Ph.D degrees in the P.G .Department of Microbiology. He has been a resource person in different National and International seminars in India and abroad. He has been the co-ordinator in the UGC sponsored programme for developing e-resources in Microbiology.

Course certificate

30% for in course Assessment & 70% of end term Proctored Exam. Securing 40% in both separately is mandatory to pass the course and get Credit Certificate.


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