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Advance Analytical Techniques

By Dr. Shahab Ali Asghar Nami, Dr. Mohammad Zain Khan   |   Aligarh Muslim University
Learners enrolled: 764
ABOUT THE COURSE:
This course offers an exciting gateway into the world of modern analytical science, providing in-depth knowledge of advanced spectroscopic and separation techniques along with their principles and instrumentations. These powerful methods are essential for studying and characterizing new materials, detecting toxicants in environmental and biological samples, and assessing the purity of raw materials and finished products. Widely used for both qualitative and quantitative analysis, these techniques forms the backbone of research laboratories, industries, and clinical diagnostic centres. The course covers a wide range of analytical tools that drive today’s innovations in materials science, pharmaceuticals, biotechnology, environmental monitoring, and medical diagnosis, making it one of the most job-oriented and skill-enhancing paper. By mastering these techniques, learners will build strong analytical capabilities that are highly valuable in industries, research institutions, and medical colleges, preparing them for myriads roles as analysts, instrument specialists, and research professionals. Designed to inspire curiosity and build real-world competence, this course ensures that students of chemical, physical, and biological sciences as well as engineering disciplines gain the essential practical skills needed to excel in modern scientific careers.

INTENDED AUDIENCE: Undergraduate, Postgraduate, Research Scholar and Industry Personnel

PREREQUISITES: Undergraduate Students

INDUSTRY SUPPORT: All Chemical and Pharmaceutical industries need experts in instrumental methods/spectroscopy so that they can effectively render their services Quality Control or R&D
Indicative Industry Sectors :
Education and Training
Note: This is an indicative list based on suggestions provided by course coordinators and should not be treated as final and binding.
Indicative Program Alignments :
M.S. B.Sc.
Summary
Course Status : Upcoming
Course Type : Elective
Language for course content : English
Duration : 12 weeks
Category :
  • Chemistry
Credit Points : 3
Level : Undergraduate/Postgraduate
Start Date : 26 Jan 2026
End Date : 30 Apr 2026
Enrollment Ends : 28 Feb 2026
Exam Date :
Translation Languages : English
NCrF Level   : 6.0 — 7.0
Industry Details : Education and Training

Page Visits



Course layout

Week 1: Introduction to spectroscopy, Electromagnetic radiation (EMR) their features and Electromagnetic spectrum, Historical background of analytical spectroscopy. Absorption and emission spectroscopy, Principle of absorption and emission spectroscopy, Instrumentation, components and their working. Atomic spectroscopy: Atomic absorption spectroscopy (AAS), History of AAS, Principle of AAS, Components of AAS. 

Week 2: Component of atomic absorption and emission spectroscopy, Calibration curve and Interferences: Spectral and Non-spectral (Matrix, Chemical, and Ionization) interferences, Applications of Atomic absorption spectroscopy. Atomic emission spectroscopy, Principle, Heat source (automizer), Instrumentation of AES, Excitation Sources: Flame excitation, AC arc, DC arc, AC spark and Plasma excitation (ICP, DCP and MWP), Advantages and disadvantages.
 
Week 3: Introduction to Inductively coupled plasma source (ICP), Sample introduction, Plasma formation and appearance, Analyte atomization and ionization, ICP-AES instrumentation. Types of spectrometers: Sequential & Simultaneous Spectrometers (Slew Scan Spectrometers & multi-channel spectrometers), Sample preparation methods, Interferences & detection limits, Application of ICP, Problems.

Week 4: Introduction to molecular luminescence, Theory of fluorescence and phosphorescence, Jablonski diagram, Internal conversion, Intersystem crossing, Delayed fluorescence, Quenching, Emission and excitation spectra.

Week 5: Relationship between concentration and fluorescence intensity, Factors affecting, Working principle and instrumentation of fluorescence and phosphorescence, Quantum efficiency, Inner filter effect, Self-quenching, Reabsorption.

Week 6: Instruments for measuring fluorescence and phosphorescence, Methods of Fluorescence Measurement, Chemiluminescence, Fluorometric determination of inorganic and organic species, Application of molecular luminescence spectroscopy, Problems.

Week 7: Introduction, classification of chromatographic methods, Elution chromatography on columns, Migration rates of solutes: distribution constants, retention time and retention factor, selectivity factor.

Week 8: Zone broadening & column efficiency, van Deemeter equation, optimization of column performance, column resolution, effect of relative and selectivity factor on resolution, effect of resolution on retention time, variables that affect column performance, problems.

Week 9: Gas Chromatography: Principle, Instruments for gas liquid chromatography, mobile phase, sample injection, gas chromatographic columns, liquid phase and column selection, classification of stationary phase.

Week 10: Detectors:  Flame ionization detector, thermal conductivity detector, Electron capture detector, thermionic detector, photoionization detector, atomic emission detector, mass-spectrometer detector and others, interpretation of gas chromatograms, temperature programming in GC, qualitative and quantitative analysis, problems.

Week 11: Liquid chromatographic methods, principle of HPLC, structural types of column packing, column efficiency in liquid chromatography, instrumentation, mobile phase, pumping systems, sample injection systems, liquid chromatographic columns.

Week 12: Detectors: Absorbance detectors, fluorescence detectors, refractive index detectors, electrochemical detectors, partition chromatography-column for bonded phase chromatography, normal phase & reversed phase chromatography, normal phase & reversed phase packing, method development in partition chromatography, column selection, mobile phase selection, application problems.

Books and references

  • Principles of Instrumental Analysis, D.A. Skoog, F.J. Holler, 
  • T.A. Nieman, Vth edn, Thomson, Brooks/Cole, 1998 
  • Instrumental Methods of Analysis, H.H. Willard, L.L. 
  • Merritt, Jr. J.A. Dean, F.A. Seatle, Jr. 7th edn, 1986. 
  • Fundamentals of Analytical Chemistry, D.A Skoog, D.M. 
  • West, F.J. Holler, S.R. Crouch, 8th edn, Thomson/Cole Singapore, 2004. 
  • Instrumental Methods of Chemical Analysis, G.W. Ewing, 
  • Vth edn, Mc-Graw Hill Book Co. 
  • Organic Spectroscopy by YR Sharma 
  • Research papers and review articles

Instructor bio

Dr. Shahab Ali Asghar Nami

Aligarh Muslim University
Dr. Shahab Ali Asghar Nami received his Master’s and Doctoral degrees in Chemistry from Aligarh Muslim University (AMU), India, marking the beginning of an academic journey defined by rigor, innovation, and unwavering scientific curiosity. Soon after earning his Ph.D., he joined the renowned Department of Inorganic and Physical Chemistry at the Indian Institute of Science (IISc), Bangalore, as a DBT Postdoctoral Fellow, an experience that sharpened his expertise in frontier inorganic and materials chemistry. His career then expanded onto the global stage when he was appointed Senior Lecturer at Taylor’s University, Malaysia, through a prestigious Twin Academic Program in partnership with the School of Biomedical Sciences, The University of Queensland, Australia. This international engagement enriched his scientific perspective and strengthened his interdisciplinary approach. Besides Dr. Nami has also served as Visiting Scientist at Dongguk University, South Korea and University of Pecs, Hungary. Returning to India, Dr. Nami served as Assistant Professor of Chemistry at the Faculty of Unani Medicine, where he played a transformative role in bridging traditional medicinal principles with contemporary Bioinorganic Chemistry. Under his guidance, several M.D. scholars successfully completed their dissertations. He later joined the Department of Chemistry at AMU as Associate Professor before moving to the newly formed Department of Industrial Chemistry, where he continues to contribute substantially to academic and research development. A distinguished Synthetic Inorganic Chemist, Dr. Nami has more recently carved a prominent niche in advanced materials chemistry. He has established a cutting-edge research laboratory devoted to the development of multifunctional hydrogels, emphasizing intelligent, flexible materials capable of detecting ultra-low biochemical signals in real time. His work stands at the forefront of next-generation technologies for wearable electronics, point-of-care diagnostics, and sophisticated biosensing platforms. With a prolific research portfolio encompassing over 80 high-impact publications, Dr. Nami has firmly established himself as a leading contributor to modern chemical sciences. He has independently secured more than ₹80 lakhs in extramural research funding from premier national agencies such as CSIR, UGC, DST, and UPCST. His vibrant mentorship has guided several scholars to the successful completion of their Ph.D. degrees. Celebrated for his engaging teaching style, conceptual clarity, and deep subject mastery, Dr. Nami brings rich experience in teaching core Chemistry and Industrial Chemistry across UG, PG, and doctoral programs. Through his research excellence, academic leadership, and visionary scientific outlook, Dr. Nami continues to shape the evolving landscape of inorganic and materials chemistry.


Dr. Mohammad Zain Khan

Dr. Mohammad Zain Khan, is a Senior Assistant Professor in Industrial Chemistry at Aligarh Muslim University (AMU), Aligarh. With a PhD in Chemistry from AMU (2011), he's taken his expertise to the global stage, completing postdoctoral work at University College London and serving as Project Scientist at IIT Delhi.
Recipient of the prestigious Erasmus Mundus Fellowship (EU) and Dr. DS Kothari Fellowship (UGC), Dr. Khan's research prowess is undeniable. He's published over 90 research articles, edited two books on environmental remediation using nanotechnology, and delivered talks at international conferences. His work has garnered over 6000 citations, with an impressive h-index of 42 and i10 index of 95.
As a dedicated educator, Dr. Khan has guided PhD students, shared his expertise, and inspired countless minds. His international exposure and experimental skills make him a standout in his field. He has been granted two patents based on his work on electrochemical sensors. With a passion for academic excellence, he's shaping the next generation of chemical scientists.
The instructors are highly qualified, holding PhD degrees in Chemical Sciences, with extensive experience in teaching and research. Their international exposure has broadened their perspectives, enriching their academic approach. They possess in-depth knowledge and hands-on experience with the techniques covered in this course, which they've applied in their own research. Additionally, they have successfully guided PhD students, demonstrating their mentorship capabilities.

Course certificate

The course is free to enroll and learn from. But if you want a certificate, you have to register and write the proctored exam conducted by us in person at any of the designated exam centres.
The exam is optional for a fee of Rs 750/- (Rupees Seven Hundred and Fifty only).
Fee for General (UR) ₹ 750/- (per course) for one course
₹ 600/- (per course) for additional course(s)
Fee for Gen-EWS / OBC-(NCL) / SC / ST / PwD / PwBD ₹ 500/- (per course) for one course
₹ 400/- (per course) for additional course(s)
Date and Time of Exams: To be declared later by NTA
Registration url: Announcements will be made when the registration form is open for registrations.
The online registration form has to be filled and the certification exam fee needs to be paid. More details will be made available when the exam registration form is published. If there are any changes, it will be mentioned then.
Please check the form for more details on the cities where the exams will be held, the conditions you agree to when you fill the form etc.

CRITERIA TO GET A CERTIFICATE

Average assignment score = 30% of average of best 8 assignments out of the total 12 assignments given in the course.
Exam score = 70% of the proctored certification exam score out of 100

Final score = Average assignment score + Exam score

Please note that assignments encompass all types (including quizzes, programming tasks, and essay submissions) available in the specific week.

YOU WILL BE ELIGIBLE FOR A CERTIFICATE ONLY IF AVERAGE ASSIGNMENT SCORE >=12/30 AND EXAM SCORE >= 28/70. If one of the 2 criteria is not met, you will not get the certificate even if the Final score >= 40/100.

Certificate will have your name, photograph and the score in the final exam with the breakup. It will have the logos of INI and AMU.

Only the e-certificate will be made available. Hard copies will not be dispatched.

Once again, thanks for your interest in our online courses and certification. Happy learning.

- INI Team
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